| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091686 |
| Chromosome: | chromosome 14 |
| Location: | 3561491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGGTTCCGCAGCTGCAGTTGCCCGTA |
| Internal bar code: | GCATAGCGTGTAGTCAGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 36.7089 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAAGCTCATCACAGCGG |
| Suggested primer 2: | CAGCTCCACAACAGGAACAA |