| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091691 |
| Chromosome: | chromosome 12 |
| Location: | 4834247 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524800 | (1 of 4) IPR005819 - Histone H5 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGTCAACACGCCGGATGAACTTTGAC |
| Internal bar code: | AATATATTTGCTCATGCGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 99.1597 |
| LEAP-Seq n confirming: | 1416 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGAGGGAACAACCCGA |
| Suggested primer 2: | TCGTGTCTGTCTAAGCACGG |