| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091714 |
| Chromosome: | chromosome 2 |
| Location: | 6261712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114300 | (1 of 6) PTHR22916//PTHR22916:SF9 - GLYCOSYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCGCACCGCCACCGACGCAGCAGCAG |
| Internal bar code: | TATTTAATCATTAGGTCGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 245 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCTGTTATTGCCAGCG |
| Suggested primer 2: | CTAATCGCGAATGATGGGTT |