| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091714 |
| Chromosome: | chromosome 3 |
| Location: | 7295332 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206950 | (1 of 1) PF05183 - RNA dependent RNA polymerase (RdRP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCAGGAAACGTTTCACACCAAAAGGG |
| Internal bar code: | ATTGGGGTATTGGTATCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 98.8701 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCGTTGTTGCTTGACAG |
| Suggested primer 2: | TTCAGTCGCATGGTGTCATT |