| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091717 |
| Chromosome: | chromosome 6 |
| Location: | 7595817 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301100 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATTGTTAGCCATCGGATGGTTTGTCT |
| Internal bar code: | CCGATGCATTAAGTGCTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 93.4928 |
| LEAP-Seq n confirming: | 13448 |
| LEAP-Seq n nonconfirming: | 936 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTTCACCACCGTCATC |
| Suggested primer 2: | CTAGACCACCCACCGACCTA |