Insertion junction: LMJ.RY0402.091737_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.091737
Chromosome:chromosome 16
Location:2895575
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g663750 (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCACTCGCCCAGAAGTAGTTCGCTATAAG
Internal bar code:GTCGCCCGGGCAACCTCACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:127
LEAP-Seq percent confirming:24.4489
LEAP-Seq n confirming:488
LEAP-Seq n nonconfirming:1508
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGTCGGTGAACACCTC
Suggested primer 2:GCGTGTATCACATCCACCTG