| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091764 |
| Chromosome: | chromosome 4 |
| Location: | 2518286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222050 | (1 of 3) K16489 - protein SFI1 (SFI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTAGTTCGTGTAACCCTGCGGCATGCTT |
| Internal bar code: | CGTCCAGGGTTTTCCGTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 80.8752 |
| LEAP-Seq n confirming: | 998 |
| LEAP-Seq n nonconfirming: | 236 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTGCACACAGGAAATA |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |