Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.091825 |
Chromosome: | chromosome 4 |
Location: | 3974333 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g231614 | (1 of 1) IPR001611//IPR003591//IPR023353 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // LemA-like domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGTCACTGCCGCTGCCACCGCCCTGC |
Internal bar code: | ATAGGGTAGTAACGCAGGACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 346 |
LEAP-Seq percent confirming: | 99.7557 |
LEAP-Seq n confirming: | 3675 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGTACGAGATGACAGGAG |
Suggested primer 2: | GGACAGTGTGGATCATCGTG |