| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091834 |
| Chromosome: | chromosome 1 |
| Location: | 4107968 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGCGCCGCTGGCGGCTGCTGCAATGG |
| Internal bar code: | CACGGGACAACCTAGTTTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 95.0459 |
| LEAP-Seq n confirming: | 518 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACGGCCACTAAACGGTA |
| Suggested primer 2: | CCGTCACTGCAGTCATTGTC |