| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091834 |
| Chromosome: | chromosome 9 |
| Location: | 3778697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391393 | (1 of 726) IPR011009 - Protein kinase-like domain | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCATGCAAGTCGCTGATAGCAGTAGTGA |
| Internal bar code: | TTGTACAAGTTTACGCGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 3.85399 |
| LEAP-Seq n confirming: | 454 |
| LEAP-Seq n nonconfirming: | 11326 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCACAAGTAAGCCAAT |
| Suggested primer 2: | TACACACAAAACCAGGCCAA |