| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091843 |
| Chromosome: | chromosome 12 |
| Location: | 6960888 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560250 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCCGAAGAAGGAGGCCTGCAAGGGGG |
| Internal bar code: | AATTGGCGGTTTGTAGTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 97.4672 |
| LEAP-Seq n confirming: | 3271 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACGCACACACAC |
| Suggested primer 2: | CCATGGCACCAGGAGTAAGT |