| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091845 |
| Chromosome: | chromosome 3 |
| Location: | 5514112 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTCTCCGTCGTATGTGCCAATGCTGCC |
| Internal bar code: | TCATGATAGTTCGGCGACTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 98.3934 |
| LEAP-Seq n confirming: | 12249 |
| LEAP-Seq n nonconfirming: | 200 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCACACTGGTTAGGAT |
| Suggested primer 2: | GTAAGCTTGGCAACAGGCTC |