| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091849 |
| Chromosome: | chromosome 1 |
| Location: | 8007302 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g070567 | (1 of 1) PTHR14440:SF7 - DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTTACCTTTAACATCGGTGCACAAAAG |
| Internal bar code: | GGATGCAGCGCCGAGCTCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1099 |
| LEAP-Seq percent confirming: | 98.6755 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATACACAAGCCATGCCAC |
| Suggested primer 2: | GTGCATAGTTTGGGGCAGTT |