| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091864 |
| Chromosome: | chromosome 6 |
| Location: | 7670768 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301650 | (1 of 1) PTHR11808//PTHR11808:SF41 - TRANS-SULFURATION ENZYME FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCAACACACGCTGACCCGCACCACCAC |
| Internal bar code: | TCGCTCGGCATCACACGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 84.953 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAGGCACGTCAGTAACA |
| Suggested primer 2: | GGAGGCGGTGTTACAGTCAT |