| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091865 |
| Chromosome: | chromosome 16 |
| Location: | 4275425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668150 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGAGGTCAAATGTGCTTGCACAGACAG |
| Internal bar code: | GGGCCACGGAAAAGGCAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 93.4211 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTACCCGACAACAACA |
| Suggested primer 2: | GTCACACACAAGGATGACCG |