Insertion junction: LMJ.RY0402.091870_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.091870
Chromosome:chromosome 8
Location:2506715
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g371957 RAPTOR1,RAPTOR Regulatory-associated protein of TOR; (1 of 1) K07204 - regulatory associated protein of mTOR (RAPTOR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGCCAGCTGACGGCGCTGCCGCCTCCCC
Internal bar code:GTCGCAGAGCCGAAGGTGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:268
LEAP-Seq percent confirming:85.9119
LEAP-Seq n confirming:3043
LEAP-Seq n nonconfirming:499
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCGGTCAGTCAGGGAGT
Suggested primer 2:TGGTTCGACTTAGTTTGGGC