| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091875 |
| Chromosome: | chromosome 7 |
| Location: | 138557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313122 | (1 of 2) 3.4.19.1 - Acylaminoacyl-peptidase / N-acylpeptide hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCTCCCGGCAGCGCCCCTCCCAGTCC |
| Internal bar code: | ATTCTAGACAGCATGCCTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 97.1074 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCATGCAGCTCTCTTGC |
| Suggested primer 2: | CTGCCAGTCTCATGTAGCCA |