Insertion junction: LMJ.RY0402.091885_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.091885
Chromosome:chromosome 1
Location:5096766
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g035450 (1 of 1) PTHR23291:SF36 - PROTEIN XBX-6, ISOFORM B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAATACTCAGACAGGGCATCCCTTCACA
Internal bar code:GAACTGAGGGTGGGACGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:275
LEAP-Seq percent confirming:32.0382
LEAP-Seq n confirming:940
LEAP-Seq n nonconfirming:1994
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAGTCCCCAAGGTTTAT
Suggested primer 2:CGCTCAACCTCTACCTCGAC