| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091904 |
| Chromosome: | chromosome 14 |
| Location: | 1283137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616600 | FZL,FZL1 | Plastid-localized; (1 of 1) PTHR11649//PTHR11649:SF36 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // FZL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCTCCGACACGCAATGTAACCAAAAA |
| Internal bar code: | CCCCGCTGAACCGCACGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 97.725 |
| LEAP-Seq n confirming: | 1933 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGAGAGCGCATTTTGTA |
| Suggested primer 2: | ACTCAAATCACTGAACCCGC |