| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091943 |
| Chromosome: | chromosome 8 |
| Location: | 4869190 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384800 | (1 of 2) 3.1.1.81 - Quorum-quenching N-acyl-homoserine lactonase / Quorum-quenching N-acyl homoserine lactone hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCACGACTCCACGCCGCATATCCCCTA |
| Internal bar code: | CCTGCTCGCTACACCCTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAAGAATCACAAGAGCC |
| Suggested primer 2: | CTCGATGCATCCCTAAGCTC |