| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091948 |
| Chromosome: | chromosome 6 |
| Location: | 7246721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298225 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGGCAGGCTCGCCAGTGCACGATGCCG |
| Internal bar code: | ATAATGCTCAAGGGCGAAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 88.2855 |
| LEAP-Seq n confirming: | 1138 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAGCAACTCTTGCAGCC |
| Suggested primer 2: | CTGTAGCACCTTGCACCTGA |