| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091951 |
| Chromosome: | chromosome 1 |
| Location: | 4910478 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034050 | (1 of 1) PTHR11751:SF428 - PROTEIN NKAT-1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCAAAGATGGTCGTTGGCAGCGACGAA |
| Internal bar code: | GCGATAGAGGGGAGTCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCCTCCCCACCTCTAC |
| Suggested primer 2: | TATCAGGTCAGCACTCACGC |