| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091962 |
| Chromosome: | chromosome 9 |
| Location: | 3310409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388319 | (1 of 1) PTHR13390:SF0 - UPF0554 PROTEIN C2ORF43 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTCATGCTGTAGGTTGTCCAATCACG |
| Internal bar code: | TAGTTTACGTCACTGGACTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.797 |
| LEAP-Seq n confirming: | 983 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTCAGTAGCAGTGCTCG |
| Suggested primer 2: | GGCGCTGTATAGCGAAAGTC |