| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091971 |
| Chromosome: | chromosome 3 |
| Location: | 4120441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCTGCCCCGCCAAGCAGGCAGTGCGCC |
| Internal bar code: | CCCGAAGGCGCTCCGAAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.0861 |
| LEAP-Seq n confirming: | 5421 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGCTAGAAGACTGGGCT |
| Suggested primer 2: | GTCCGACTTCCAAGCTTACG |