Insertion junction: LMJ.RY0402.091981_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.091981
Chromosome:chromosome 11
Location:2467456
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGCCCCACGCTGCAAGGTAGGGTAGGG
Internal bar code:ACAACGTCGATCTTTTGTGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:84.7322
LEAP-Seq n confirming:3907
LEAP-Seq n nonconfirming:704
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATCAAGGAGCACAGACA
Suggested primer 2:ACTGCATCGAATGCACTGAG