Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.091981 |
Chromosome: | chromosome_11 |
Location: | 2467456 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g475600 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCAGCCCCACGCTGCAAGGTAGGGTAGGG |
Internal bar code: | ACAACGTCGATCTTTTGTGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 183 |
LEAP-Seq percent confirming: | 84.7322 |
LEAP-Seq n confirming: | 3907 |
LEAP-Seq n nonconfirming: | 704 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAATCAAGGAGCACAGACA |
Suggested primer 2: | ACTGCATCGAATGCACTGAG |