| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092021 |
| Chromosome: | chromosome 16 |
| Location: | 2126319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657950 | SSA5 | cilia-sensing, structure and/or assembly; (1 of 1) K06259 - CD36 antigen (CD36) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGTGGTGTGGTGTGGTGTGGTGTTGT |
| Internal bar code: | TCTTCGCTCGCGCGGTGCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 95.4855 |
| LEAP-Seq n confirming: | 1121 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATGAAAGAGCGAGGAG |
| Suggested primer 2: | CTACAGCCCCTTGAGAGTGC |