Insertion junction: LMJ.RY0402.092036_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.092036
Chromosome:chromosome 13
Location:4858433
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g605600 OAS1 (1 of 1) IPR018952//IPR026774 - 2'-5'-oligoadenylate synthetase 1, domain 2/C-terminal // 2'-5'-oligoadenylate synthase; 2'-5'-oligoadenylate synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGATCTGATACATGCCCTTACATGCCTG
Internal bar code:GGATCATGAAATTATTCCATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1034
LEAP-Seq percent confirming:99.6576
LEAP-Seq n confirming:22409
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ATATATCCCCATTCACCGCA
Suggested primer 2:CCTATCTGCCACGTAACGGT