Insertion junction: LMJ.RY0402.092045_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092045
Chromosome:chromosome 4
Location:495267
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCACCCTATGCTAGGAGTTCAGTGGGTG
Internal bar code:ACTAAACATATGGGTAATGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:64.411
LEAP-Seq n confirming:257
LEAP-Seq n nonconfirming:142
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCCATTTCTTGACGTGC
Suggested primer 2:ACCCTCTACAACCCAGCCTT