| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092062 |
| Chromosome: | chromosome_10 |
| Location: | 1290780 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g427300 | RSP2 | Radial spoke protein 2 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTGTTTGGGTGTTCAACACGCATATGCC |
| Internal bar code: | TAGGGCGATAGACCTTATATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1238 |
| LEAP-Seq percent confirming: | 99.5422 |
| LEAP-Seq n confirming: | 6305 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGCTAGTAGGATGACGG |
| Suggested primer 2: | TTTACAATCCCTCAGGACGC |