| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092066 |
| Chromosome: | chromosome 8 |
| Location: | 4627832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383450 | FIST N domain protein; (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCACGCCGGCCCGGTGGCCTTGGGTCC |
| Internal bar code: | CGGCGGATGCGTGGTCGGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 92.8444 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTGGGTGGAGTTTTGA |
| Suggested primer 2: | CTACGCTCTCGACCCTGAAC |