| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092078 |
| Chromosome: | chromosome 16 |
| Location: | 7285025 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683483 | REX1-B,REX1B | (1 of 1) PF14966 - DNA repair REX1-B (DNA_repr_REX1B); DNA repair protein and protein of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACACAAGGCCTTCATGCGGAACAAGTGA |
| Internal bar code: | TGGTTGAATGTCATAATCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 97.6546 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGACAGACAAGTGGCAGA |
| Suggested primer 2: | GCGTTCACGGGTTTACAACT |