Insertion junction: LMJ.RY0402.092088_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.092088
Chromosome:chromosome 3
Location:9199859
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g213201 OPR108,CCS2 Cytochrome c assembly facor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCGCCGTCGGCCTGGCTTGCCGCCCCCA
Internal bar code:ACGGGGGGAACGCGCCATGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:125
LEAP-Seq percent confirming:97.493
LEAP-Seq n confirming:350
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGAGCTTGACCCTTCGTT
Suggested primer 2:CAGTAGCACGCCCAGTCATA