| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092115 |
| Chromosome: | chromosome 3 |
| Location: | 707583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146027 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCAAACCCACCTGCGCGCATACCGACT |
| Internal bar code: | GCTGGGCCGTATAAGTCGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.8925 |
| LEAP-Seq n confirming: | 15537 |
| LEAP-Seq n nonconfirming: | 174 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCGACTACTTCGCATCG |
| Suggested primer 2: | GCACGCGCTAGTTATTGACA |