| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092164 |
| Chromosome: | chromosome 12 |
| Location: | 3046232 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500650 | RNB2 | (1 of 1) PTHR23355//PTHR23355:SF39 - RIBONUCLEASE // SUBFAMILY NOT NAMED; chloroplast RNase II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCCCTCCGCTCCTCCCCTCCGCTCC |
| Internal bar code: | GCTGATCATCGGTTTACGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3 |
| LEAP-Seq percent confirming: | 97.7011 |
| LEAP-Seq n confirming: | 255 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACTTCCGAAAACCCTG |
| Suggested primer 2: | ATCCTCAAATCCAATGCTGC |