Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.092168 |
Chromosome: | chromosome 7 |
Location: | 5534603 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g351300 | (1 of 2) PTHR11451//PTHR11451:SF41 - TRNA SYNTHETASE-RELATED // GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGGGGGGCGAAGGGCAGCGCAAGAAGA |
Internal bar code: | TAAGTCCTCCGGTAGTCGCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 138 |
LEAP-Seq percent confirming: | 55.0708 |
LEAP-Seq n confirming: | 467 |
LEAP-Seq n nonconfirming: | 381 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGCCGTTCCTCAAATGTA |
Suggested primer 2: | CACATAGGTCTTGCCCTGGT |