| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092195 |
| Chromosome: | chromosome 3 |
| Location: | 3612425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168550 | AOT3 | Amino acid transporter; (1 of 1) K15015 - solute carrier family 32 (vesicular inhibitory amino acid transporter) (SLC32A, VGAT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCGGCCAGTCGGTCAGCCGGCTGTGC |
| Internal bar code: | GTTCCCAAAGGTGTTAGATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 99.7191 |
| LEAP-Seq n confirming: | 1420 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGTGGAGATGGAGGA |
| Suggested primer 2: | GTCTTGACAGGTGCGTCTGA |