| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092195 |
| Chromosome: | chromosome 11 |
| Location: | 3508814 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481350 | (1 of 14) PF14295 - PAN domain (PAN_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCATTATACGGTCGTCGTCACTACGGG |
| Internal bar code: | AGACGGGGTCAGAGCGAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 62.6214 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 693 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAACACAACAGGTCCCC |
| Suggested primer 2: | GAGCTCCTTTGACTTGCCAC |