Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.092208 |
Chromosome: | chromosome_11 |
Location: | 3607680 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g482001 | FAP208 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGTGTGTGTGTTTGTGCGTCGTTTGTGGC |
Internal bar code: | ACGTGAGCCACACCGAAGGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 461 |
LEAP-Seq percent confirming: | 94.0778 |
LEAP-Seq n confirming: | 1112 |
LEAP-Seq n nonconfirming: | 70 |
LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGGTTGTGTGGTTGTGTG |
Suggested primer 2: | AAAGGTTGGTAAGGGGTTGG |