Insertion junction: LMJ.RY0402.092216_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.092216
Chromosome:chromosome 7
Location:747062
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g317800 (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCATCAAGGGGGGAGTGATCGCTCTCT
Internal bar code:ATCGAAGGAAACTTCTAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3622
LEAP-Seq percent confirming:74.118
LEAP-Seq n confirming:4307
LEAP-Seq n nonconfirming:1504
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCTTACTCCTCGACTCAC
Suggested primer 2:CTCCCTTAACCATCCCCAAT