| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092231 |
| Chromosome: | chromosome 2 |
| Location: | 1360724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083065 | (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTGGCCCAGGGTTCGTGCCTTCGCGC |
| Internal bar code: | CGAAATAACGAATCTGGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 81.1307 |
| LEAP-Seq n confirming: | 49308 |
| LEAP-Seq n nonconfirming: | 11468 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTAGCACGGAGACTGG |
| Suggested primer 2: | ACGTACACGAAGTGCGAGTG |