| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092270 |
| Chromosome: | chromosome 7 |
| Location: | 5307206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349540 | (1 of 1) PF07714//PF13857 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (many copies) (Ank_5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTGCGTTCCTTTTGTGGCGTGCACGT |
| Internal bar code: | GGATAGGTCCCGTCGGCCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 98.6027 |
| LEAP-Seq n confirming: | 63582 |
| LEAP-Seq n nonconfirming: | 901 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGCTGGGTGCTTTACC |
| Suggested primer 2: | TCACGTGAGTGACAGCATCA |