| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092272 |
| Chromosome: | chromosome 1 |
| Location: | 3300768 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020850 | CYG49 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGCGCTGGGACTCAGTGGCCGCGGTG |
| Internal bar code: | AAAACACATGTCAGGTGCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 70.5581 |
| LEAP-Seq n confirming: | 1239 |
| LEAP-Seq n nonconfirming: | 517 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCCATTTCTAGCCCAGC |
| Suggested primer 2: | TCTGTGTTATCTTCCGTGCG |