| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092301 |
| Chromosome: | chromosome 12 |
| Location: | 3192164 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499152 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCTCGCAACCGTGTGCGCGAACGTGTA |
| Internal bar code: | GTTAGCAATTCGTCGGTAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 53.8708 |
| LEAP-Seq n confirming: | 5880 |
| LEAP-Seq n nonconfirming: | 5035 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCAACCGTCCAACAAAG |
| Suggested primer 2: | GCGAACCGCTACCTAGACAC |