| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092303 |
| Chromosome: | chromosome 16 |
| Location: | 2310850 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659300 | CYB2,CYB5-2 | (1 of 2) PTHR19359//PTHR19359:SF29 - CYTOCHROME B5 // SUBFAMILY NOT NAMED; Cytochrome b5 flavohemoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTGTGCAGTCTGCCGTTCACAGCGTA |
| Internal bar code: | GCCTGAGCGATCTTTTAACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 96.7146 |
| LEAP-Seq n confirming: | 471 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCTTGAGCTCGCTTCT |
| Suggested primer 2: | TCGGTGGGAGCTTAAACAAC |