| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092305 |
| Chromosome: | chromosome 17 |
| Location: | 2060241 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711750 | HTB5,HTB22 | Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGATGCTCAGCTGTGTACGTGCACGCC |
| Internal bar code: | GGTGCCGGCTTGTGGCTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 99.7583 |
| LEAP-Seq n confirming: | 2064 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACACCACCCTCAACCT |
| Suggested primer 2: | CAAGTTCACCTCGGGCTAAG |