| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092314 |
| Chromosome: | chromosome 4 |
| Location: | 2065873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219100 | (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGGTGAACGGCCTAATTGATCCATGA |
| Internal bar code: | CCGTGAAAGCCGAGGATGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.2234 |
| LEAP-Seq n confirming: | 3194 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTCCTGCACTTCCTGCT |
| Suggested primer 2: | ACGACAGCCACATCCTAACC |