| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092343 |
| Chromosome: | chromosome 10 |
| Location: | 6049465 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463250 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAACATCAAGGCACGGCACAGGGCCA |
| Internal bar code: | TATTTATGCAGATTCTGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 58.6454 |
| LEAP-Seq n confirming: | 2208 |
| LEAP-Seq n nonconfirming: | 1557 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAACCCTGCTGTGCACTC |
| Suggested primer 2: | ATGACTGTAGGTATGCGCCC |