| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092353 |
| Chromosome: | chromosome 10 |
| Location: | 129737 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418600 | RLS3 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCGGGAGGAAGGGAAATGGTCAGTGC |
| Internal bar code: | GCGATTCAATCGTGCTTCCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 99.4782 |
| LEAP-Seq n confirming: | 5529 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAATTCTGTGCCCACTCC |
| Suggested primer 2: | GTGCAGGCAGGCTAGATAGG |