| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092365 |
| Chromosome: | chromosome 1 |
| Location: | 7399254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053200 | (1 of 1) PTHR12415:SF0 - TYROSYL-DNA PHOSPHODIESTERASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTACGACGTAAACACACCCATGCAGC |
| Internal bar code: | GGCGCGTTTCGTCCACCCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 98.8543 |
| LEAP-Seq n confirming: | 2761 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCACCACCACCTCTACC |
| Suggested primer 2: | CATGTAGTTGGACACCAGCG |