| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092425 |
| Chromosome: | chromosome 10 |
| Location: | 1883675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431650 | (1 of 2) 2.7.1.86 - NADH kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTCGTGCACGTGGTTCGGTGGGCTTTA |
| Internal bar code: | TGGGTGTCTCGAGGAGGGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 98.9744 |
| LEAP-Seq n confirming: | 1930 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCATGTTTGTTTCCCACC |
| Suggested primer 2: | AGCAACCTAGGACCTGAGCA |